Anti-Complement C3a/C3a (desArg) [Z22/8] Antibody
![Anti-Complement C3a/C3a (desArg) [Z22/8] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1220_2001202001263912190.jpg "Anti-Complement C3a/C3a (desArg) [Z22/8] Antibody")
This mouse IgG2b/k monoclonal antibody was generated against purified porcine complement C3a and recognizes porcine complement C3a/C3a (desArg).
Highlights:
- Specific for porcine C3a/C3a (desArg)
- Useful as capture antibody in C3a ELISA
- Blocks C3a biological activity
- Suitable for Western Blot, ELISA, Immunoprecipitation applications, as well as functional studies
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
This mouse IgG2b/k monoclonal antibody was generated against purified porcine complement C3a and recognizes porcine complement C3a/C3a (desArg).
Highlights:
- Specific for porcine C3a/C3a (desArg)
- Useful as capture antibody in C3a ELISA
- Blocks C3a biological activity
- Suitable for Western Blot, ELISA, Immunoprecipitation applications, as well as functional studies
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
| Product Type: | Antibody |
| Antigen: | Porcine C3a/C3a (desArg) |
| Accession ID: | Q16581 |
| Isotype: | IgG2b/k |
| Clonality: | Monoclonal |
| Clone Name: | Z22-8 |
| Reactivity: | Porcine |
| Immunogen: | Purified porcine C3a |
| Species Immunized: | Mouse (Balb/c) |
| Epitope: | Activation-dependent neo-epitope present on porcine C3a, but not C3 |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, ELISA, IP, and Functional Studies |
| Storage: | -20C |
| Shipped: | Cold packs |
| Clone | Reactivity | Isotype | Epitope | Applications | Functional Properties |
| M20/6 | Human Ba / Factor B | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in Ba ELISA (together with D22/3) |
| D22/3 | Human Ba | IgG2b/k | Neo-Epitope on Ba (Glu215, Arg234) | WB, ELISA, Flow Cytometry | Capture Ab in Ba ELISA (together with M20/6) |
| I3/15 | Human and baboon activated C3 | IgG1k | Neo-Epitope on C3b/iC3b/C3dg | WB, ELISA | Capture Ab in actC3 ELISA (together with rab anti-C3dg) |
| Z22/8 | Porcine C3a/C3a(desArg) | IgG2b/k | Neo-Epitope on C3a/C3a(desArg) | WB, ELISA, IP | Capture Ab in C3a ELISA (together with K5/9); blocks C3a biological activity |
| K5/9 | Porcine C3a/C3a(desArg) | IgG1k | N/A | WB, ELISA | Capture Ab in C3a ELISA (together with Z22/8); blocks C3a biological activity |
| G25/2 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in C5a ELISA (together with C17/5); blocks C5a biological activity |
| C17/5 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a/C5a(desArg) | WB, ELISA | Capture Ab in C5a ELISA (together with G25/2); blocks C5a biological activity |
| T13/9 | Porcine C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a (Tyr57- Gly74) | WB, ELISA, IP | Capture Ab in C5a ELISA (together with rab anti-C5a); blocks C5a biological activity |
- Oppermann, M., Höpken, U., and Götze, O. (1992) Immunopharm. 24, 119-134
- Höpken, U., Strüber, A., Oppermann, M., Mohr, M., Mücke, K. H., Burchardi, H., and Götze, O. (1993) Production and characterization of peptide-specific monoclonal antibodies that recognize a neoepitope on hog C5a. In Faist, E., Meakins, J., and Schildberg, F. W., editors. Host defense dysfunction in trauma, shock and sepsis, Berlin,Heidelberg, Springer Verlag.
- Höpken, U., Mohr, M., Struber, A., Montz, H., Burchardi, H., Götze, O., and Oppermann, M. (1996) Eur. J. Immunol. 26, 1103-1109
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