Anti-Complement C5/C5a/C5a (desArg) [G25/2] Antibody
![Anti-Complement C5/C5a/C5a (desArg) [G25/2] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1219_2001202001263912180.jpg "Anti-Complement C5/C5a/C5a (desArg) [G25/2] Antibody")
This mouse IgG1k monoclonal antibody was generated against purified human C5a and recognizes human and baboon complement C5/C5a/C5a (desArg).
Highlights:
- Reacts with human and baboon C5/C5a/C5a (desArg)
- Useful as detecting antibody in C5a ELISA
- Blocks C5a biological activity
- Suitable for Western Blot, ELISA, Immunoprecipitation applications, as well as functional studies
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
This mouse IgG1k monoclonal antibody was generated against purified human C5a and recognizes human and baboon complement C5/C5a/C5a (desArg).
Highlights:
- Reacts with human and baboon C5/C5a/C5a (desArg)
- Useful as detecting antibody in C5a ELISA
- Blocks C5a biological activity
- Suitable for Western Blot, ELISA, Immunoprecipitation applications, as well as functional studies
The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
Specifications
| Product Type: | Antibody |
| Antigen: | Human C5a/C5a (desArg) |
| Accession ID: | P01031 |
| Isotype: | IgG1k |
| Clonality: | Monoclonal |
| Clone Name: | G25-2 |
| Reactivity: | Human, baboon |
| Immunogen: | Purified human C5a |
| Species Immunized: | Mouse (Balb/c) |
| Epitope: | N/A |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | WB, ELISA, IP, and Functional Studies |
| Storage: | -20C |
| Shipped: | Cold packs |
Research
| Clone | Reactivity | Isotype | Epitope | Applications | Functional Properties |
| M20/6 | Human Ba / Factor B | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in Ba ELISA (together with D22/3) |
| D22/3 | Human Ba | IgG2b/k | Neo-Epitope on Ba (Glu215, Arg234) | WB, ELISA, Flow Cytometry | Capture Ab in Ba ELISA (together with M20/6) |
| I3/15 | Human and baboon activated C3 | IgG1k | Neo-Epitope on C3b/iC3b/C3dg | WB, ELISA | Capture Ab in actC3 ELISA (together with rab anti-C3dg) |
| Z22/8 | Porcine C3a/C3a(desArg) | IgG2b/k | Neo-Epitope on C3a/C3a(desArg) | WB, ELISA, IP | Capture Ab in C3a ELISA (together with K5/9); blocks C3a biological activity |
| K5/9 | Porcine C3a/C3a(desArg) | IgG1k | N/A | WB, ELISA | Capture Ab in C3a ELISA (together with Z22/8); blocks C3a biological activity |
| G25/2 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | N/A | WB, ELISA, IP | Detecting Ab in C5a ELISA (together with C17/5); blocks C5a biological activity |
| C17/5 | Human and baboon C5/C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a/C5a(desArg) | WB, ELISA | Capture Ab in C5a ELISA (together with G25/2); blocks C5a biological activity |
| T13/9 | Porcine C5a/C5a(desArg) | IgG1k | Neo-Epitope on C5a (Tyr57- Gly74) | WB, ELISA, IP | Capture Ab in C5a ELISA (together with rab anti-C5a); blocks C5a biological activity |
Provider
From the laboratory of Martin Oppermann, MD, University Medical Center Göttingen.
References
- Oppermann, M., Kurts, C., Zierz, R., Quentin, E., Weber, M. H., and Götze, O. (1991) Kidney Int. 40, 939-947
- Oppermann, M., Höpken, U., and Götze, O. (1992) Immunopharm. 24, 119-134
- Oppermann, M., Haubitz, M., Quentin, E., and Götze, O. (1988) Klin. Wochenschr. 66, 857-864
- Nezlin, R., Freywald, A., and Oppermann, M. (1993) Mol. Immunol. 30, 935-940
- Ammon, H. P. T., Ege, W., Oppermann, M., Göpel, W., and Eisele, S. (1995) Anal. Chem. 67, 466-471
- Püschel, G. P., Dieter, P., Muschol, W., Oppermann, M., Hülsmann, M., Schulze-Specking, A., Götze, O., Decker, K., and Jungermann, K. (1991) Prostanoid production in Kupffer cells: A step in the increase in glucose and lactate output and reduction of flow by anaphylatoxins in perfused rat liver. In Wisse, E., Knook, D. L., and Decker, K., editors. Cells of the hepatic sinusoid, Leiden, Kupffer Cell Foundation
- Lhotta, K., Würzner, R., Kronenberg, F., Oppermann, M., and Konig, P. (1998) Kidney Int. 53, 1044-1051
- Oppermann, M., Schulze, M., Götze, O. (1991) A sensitive enzyme immunoassay for the quantitation of human C5a/C5a(des Arg) anaphylatoxin using a monoclonal antibody with specificity for a neotope.Complement Inflamm. 8(1):13-24
- Mollnes et al. (1993) Clin Exp Immunol 91,295-300
- Scholl et al. (2008) PLoS ONE. 3(7):e2593
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